9J56
Functional Investigation of the SAM-Dependent Methyltransferases Rdmb in Anthracycline Biosynthesis
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 79.997, 79.997, 234.203 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.719 - 2.100 |
| Rwork | 0.214 |
| R-free | 0.25880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.750 | 2.175 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.016 | |
| Number of reflections | 49588 | 5052 |
| <I/σ(I)> | 20.1 | |
| Completeness [%] | 95.5 | |
| Redundancy | 2 | |
| CC(1/2) | 1.000 | 0.914 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 289 | 0.1 M BIS-TRIS pH 6.5, 2.0 M Ammonium sulfate |
