9J54

Crystal structure of FIP200 Claw in complex with ATG16L1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-12-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	58.701, 34.894, 60.727
Unit cell angles	90.00, 118.38, 90.00

Refinement procedure

Resolution	22.460 - 1.610
R-factor	0.1797
Rwork	0.178
R-free	0.21570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.266
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.19rc4_4035)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.430	1.640
High resolution limit [Å]	1.610	1.610
Rpim	0.021	0.290
Number of reflections	27862	1383
<I/σ(I)>	17.2
Completeness [%]	98.0
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	10% v/v 2-Propanol, 0.1 M BICINE (pH 8.5), 30% w/v Polyethylene glycol 1500