9IPT

Crystal structure of a TetR family regulator AmvR from Acinetobacter baumannii with spermidine bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2023-12-02
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	60.005, 65.510, 101.032
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.950 - 2.500
R-factor	0.2304
Rwork	0.229
R-free	0.26950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.457
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.560
High resolution limit [Å]	2.500	2.500
Rmerge	0.173	1.386
Rpim	0.054	0.496
Number of reflections	14340	1022
<I/σ(I)>	20.65	1.7
Completeness [%]	100.0
Redundancy	11.43
CC(1/2)	0.953	0.573

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	0.2 M Sodium formate, 10% v/v Ethylene glycol, 20% w/v PEG 3350