9IGM

Structure of the 4-CHRD domain of human Chordin in complex with a Heparin Oligosaccharide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-22
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9537
Spacegroup name	P 62 2 2
Unit cell lengths	172.774, 172.774, 129.411
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	74.810 - 2.920
R-factor	0.2411
Rwork	0.239
R-free	0.27420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.789
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	97.880	3.100
High resolution limit [Å]	2.920	2.920
Rmerge	0.233
Rpim		0.803
Number of reflections	25297	3999
<I/σ(I)>	17.7	1
Completeness [%]	100.0	100
Redundancy	78.9	81.3
CC(1/2)	1.000	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277.15	0.1M Imidazole/MES buffer pH 6.5, 0.06M Magnesium chloride hexahydrate/Calcium chloride dihydrate, 30% Glycerol/PEG 4000. (Morpheus condition A3). 50ug/mL Heparin Dp4.