9I2N
Crystal structure of zebrafish S100I1 protein in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-07 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.380, 131.010, 45.820 |
| Unit cell angles | 90.00, 90.09, 90.00 |
Refinement procedure
| Resolution | 45.820 - 1.500 |
| R-factor | 0.1697 |
| Rwork | 0.168 |
| R-free | 0.20030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.039 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.570 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmeas | 0.054 | 0.750 |
| Number of reflections | 61980 | 7421 |
| <I/σ(I)> | 22.85 | |
| Completeness [%] | 96.0 | 90.3 |
| Redundancy | 6.8 | 6.09 |
| CC(1/2) | 1.000 | 0.830 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M Li sulfate, 0.1 M Tris-HCl pH 8.5, 25% PEG 4000 |
