9I0B

E3 ubiquitin ligase CBL-B in complex with inhibitor

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-24
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98011
Spacegroup name	P 21 21 21
Unit cell lengths	97.770, 57.260, 74.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.480 - 1.661
R-factor	0.263
Rwork	0.262
R-free	0.28010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.870
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AMoRE
Refinement software	BUSTER (2.11.8 (24-FEB-2021))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	97.770	1.808
High resolution limit [Å]	1.661	1.661
Rmerge	0.090	0.735
Rmeas	0.099	0.886
Rpim	0.040	0.482
Total number of observations	214279	5448
Number of reflections	36120	1807
<I/σ(I)>	11	1.5
Completeness [%]	71.7
Redundancy	5.9	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	9 % PEG8000, 0.05 M Mg Acetate, 2.5 % MPD, 0.05 M PCPT pH 8.0