9I0A

CARM1 in complex with arg-aDMA analog

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	75.237, 98.676, 208.551
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.330 - 2.220
R-factor	0.1922
Rwork	0.190
R-free	0.23690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.658
Data reduction software	HKL-2000 (2.3.15)
Data scaling software	Aimless (0.7.8)
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.330	2.270
High resolution limit [Å]	2.220	2.220
Rmerge	0.123	1.940
Rmeas	0.128
Rpim	0.036
Number of reflections	74004	1576
<I/σ(I)>	12.6	1.6
Completeness [%]	95.6	33.7
Redundancy	11.8	9.2
CC(1/2)	0.999	0.664

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	10% PEG3350, 100 mM tris pH7.5, 0.1M NaCl