9HYT
Crystal structure of the GFRaL receptor in complex with an inhibitory cyclic peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-24 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.980 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 59.541, 148.183, 198.053 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.000 - 1.900 |
| Rwork | 0.194 |
| R-free | 0.24450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.649 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.190 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 38664 | 11748 |
| <I/σ(I)> | 11.2 | |
| Completeness [%] | 92.5 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.999 | 0.669 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.3 M (NH4)2SO4, 0.1 M Na-citrate, 0.9 M LiSO4 100nL GFRaL-peptide mixture mixed with 80nL well solution and 20nL seed stock. |
