9HVM
In-cell Structure of Pyrenoid Rubisco
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SUBTOMOGRAM AVERAGING |
| Resolution (Å) | 8.1 |
| Software | RELION,CTFFIND |
| Symmetry | D4 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
