9HQ5

Crystal structure of Tetraspanin CD63mutant Large Extracellular Loop (LEL) in complex with sybody SB3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.984002
Spacegroup name	P 43 21 2
Unit cell lengths	65.230, 65.230, 138.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.500 - 2.600
R-factor	0.2473
Rwork	0.245
R-free	0.29170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.404
Data reduction software	XDS (202440712)
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.500	2.693
High resolution limit [Å]	2.600	2.600
Number of reflections	9778	940
<I/σ(I)>	19.94	4.88
Completeness [%]	99.8	99.37
Redundancy	10.4
CC(1/2)	0.998	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	200 mM Ammonium sulfate, 20.00 % w/v Polyethylene glycol 3,350