9HO5

Crystal Structure of the Human Frataxin protein in complex with a tailored Camelid Nanobody 4A7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97926
Spacegroup name	P 21 21 21
Unit cell lengths	38.523, 48.151, 111.425
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.710 - 1.500
R-factor	0.1878
Rwork	0.187
R-free	0.20990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.845
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21rc1_4958)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.713	1.327
High resolution limit [Å]	1.246	1.246
Number of reflections	47731	2387
<I/σ(I)>	23.6
Completeness [%]	89.3
Redundancy	10.7
CC(1/2)	0.999	0.603

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M Sodium Thiocyanate pH 6.9 20% PEG 3350