9HN3
Mouse phosphomannomutase 2 in apo state from crystals with PEG 3350
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 71.811, 71.811, 355.897 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 62.190 - 1.490 |
| R-factor | 0.1799 |
| Rwork | 0.179 |
| R-free | 0.19520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.710 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 177.950 | 1.510 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rpim | 0.019 | 0.627 |
| Number of reflections | 89547 | 4298 |
| <I/σ(I)> | 16.7 | 1.3 |
| Completeness [%] | 99.3 | 98.2 |
| Redundancy | 19.2 | 19.5 |
| CC(1/2) | 0.999 | 0.591 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | Best crystals appeared after 2-7 days using 0.1 M Tris-HCl pH 8.5, 0.2 M MgCl2, and 27-30% PEG 3350 as reservoir, in hanging drop 24-well plates, by mixing 2 ul of protein with 2 ul of reservoir solution |
