9HMI

Crystal structure of the Calf domains of Integrin Alpha5 in complex with angiopoietin2 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.99
Spacegroup name	P 21 21 21
Unit cell lengths	49.977, 69.231, 137.828
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.840 - 2.780
R-factor	0.203
Rwork	0.204
R-free	0.30600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.757
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.840	2.930
High resolution limit [Å]	2.780	2.780
Rmerge	0.200	1.600
Rmeas	0.210	0.900
Rpim	0.060	0.900
Number of reflections	12325	1511
<I/σ(I)>	7.3	0.4
Completeness [%]	97.6
Redundancy	4.4
CC(1/2)	0.997	0.150

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M ammonium formate; 20% polyethylene glycol 3350 (wt/vol)