9HMH
Crystal structure of the Calf domains of Integrin Alpha5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-01 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.016, 71.437, 121.266 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.280 - 1.920 |
| Rwork | 0.222 |
| R-free | 0.28220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.944 |
| Data reduction software | autoPROC |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.280 | 2.050 |
| High resolution limit [Å] | 1.920 | 1.970 |
| Rmerge | 1.620 | |
| Rmeas | 0.150 | |
| Number of reflections | 33875 | 7412 |
| <I/σ(I)> | 5.47 | 0.8 |
| Completeness [%] | 99.5 | 91 |
| Redundancy | 19.8 | |
| CC(1/2) | 0.995 | 0.140 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1M Bistris pH 5.5, 0.2M MgCl2, 25% PEG 3350 (wt/vol) |
