9HIC
K166 acetylated human muscle pyruvate kinase, isoform M2 (PKM2), in complex with FBP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-08 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.998, 147.230, 97.969 |
| Unit cell angles | 90.00, 103.72, 90.00 |
Refinement procedure
| Resolution | 49.380 - 2.900 |
| R-factor | 0.19965 |
| Rwork | 0.197 |
| R-free | 0.25205 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.249 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.380 | 3.004 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 46401 | 4616 |
| <I/σ(I)> | 8.03 | |
| Completeness [%] | 99.8 | |
| Redundancy | 7.1 | |
| CC(1/2) | 0.992 | 0.592 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2M MgCl2 0.1M Bis-Tris pH 5.5 25% PEG 3350 |
