9HI2
STRUCTURE OF HUMAN UDP-GALACTOSE 4-EPIMERASE IN COMPLEX WITH COVALENT COMPOUND WBC10
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-07-04 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.82 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 50.040, 114.140, 130.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.070 - 1.370 |
| R-factor | 0.1423 |
| Rwork | 0.141 |
| R-free | 0.17430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.480 |
| Data reduction software | DIALS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.070 | 1.390 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Number of reflections | 77616 | 4002 |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 99.0 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.300 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 22.7% PEG 3350 0.2 M NaSO4 0.1 Bis-Tris propane pH 7 |
