9HDH
Structure of s.c.Osh6 in complex with Ist2 732-761 and POPS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 61.642, 176.251, 95.714 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.420 - 1.840 |
| Rwork | 0.179 |
| R-free | 0.20970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1 5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.420 | 1.950 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.190 | 1.541 |
| Number of reflections | 44913 | 12172 |
| <I/σ(I)> | 7.91 | 1.38 |
| Completeness [%] | 97.9 | 87.1 |
| Redundancy | 6.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 293 | 100mM BisTris pH 5.5, 200mM KCl, 20mM MgCl2, 28% - 30% PEG400 |
