9HAQ
Crystal structure of methionine gamma-lyase from Brevibacterium sandarakinum in complex with PLP and norleucine at pH 8.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-10 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 113.832, 151.945, 316.557 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 136.980 - 2.693 |
| R-factor | 0.2591 |
| Rwork | 0.258 |
| R-free | 0.27820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.920 |
| Data reduction software | XDS (20210323) |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 109.612 | 2.930 |
| High resolution limit [Å] | 2.693 | 2.693 |
| Rmerge | 0.225 | 1.962 |
| Number of reflections | 120231 | 6012 |
| <I/σ(I)> | 10.9 | 1.6 |
| Completeness [%] | 95.9 | 72.1 |
| Redundancy | 13.7 | 12.9 |
| CC(1/2) | 0.997 | 0.520 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M Sodium acetate, 0.1 M Tris-HCl pH 8.5, 28% PEG4000, 5 mM Norleucine, 1 mM PLP, cryoprotection: 10% PEG400 |
