9H2D
Human IFT172 C-terminal U-box domain crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.006 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.967, 90.507, 67.423 |
| Unit cell angles | 90.00, 97.42, 90.00 |
Refinement procedure
| Resolution | 46.573 - 2.097 |
| R-factor | 0.1917 |
| Rwork | 0.189 |
| R-free | 0.23830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.952 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.573 | 2.100 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rpim | 0.037 | 0.483 |
| Number of reflections | 34038 | 1631 |
| <I/σ(I)> | 9.7 | 1.3 |
| Completeness [%] | 99.5 | 96.2 |
| Redundancy | 6.6 | 6.4 |
| CC(1/2) | 0.999 | 0.777 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 0.2 M Sodium phosphate dibasic dihydrate, 20% w/v Polyethylene glycol 3350, pH 9.1 |
