9H0B
Crystal structure of the Porcine Hemagglutinating Encephalomyelitis Virus (PHEV) receptor binding domain in complex with porcine DPEP1.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-31 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 96.720, 73.010, 136.620 |
| Unit cell angles | 90.00, 91.94, 90.00 |
Refinement procedure
| Resolution | 48.330 - 2.250 |
| R-factor | 0.1999 |
| Rwork | 0.198 |
| R-free | 0.23480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.750 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.330 | 2.280 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.238 | |
| Number of reflections | 90076 | 6350 |
| <I/σ(I)> | 8.4 | 1 |
| Completeness [%] | 99.6 | 95.7 |
| Redundancy | 6.9 | 6.7 |
| CC(1/2) | 0.984 | 0.454 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 0.1 M MES (pH 6.0), 0.2 M zinc acetate, 10% w/v PEG 8000 |
