9GY3
Crystal structure of CRBNmidi in complex with (S)-dHTC1
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-20 |
| Detector | DECTRIS EIGER2 X CdTe 9M |
| Wavelength(s) | 0.6199 |
| Spacegroup name | P 31 |
| Unit cell lengths | 53.501, 53.501, 236.121 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.130 - 2.500 |
| R-factor | 0.2465 |
| Rwork | 0.244 |
| R-free | 0.30320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.565 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158-000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 236.120 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 26206 | 2917 |
| <I/σ(I)> | 3.5 | |
| Completeness [%] | 98.3 | |
| Redundancy | 10.7 | |
| CC(1/2) | 0.969 | 0.182 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Morpheus H10 (0.1 M Buffer System 3 pH 8.5, 0.12 M Alcohols, 30 % Precipitant Mix 2) (Molecular Dimensions) |
