9GP4

Jumonji domain-containing protein 2A with crystallization epitope mutations Q953E:A958D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.916
Spacegroup name	I 41 3 2
Unit cell lengths	136.100, 136.100, 136.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.624 - 1.590
Rwork	0.161
R-free	0.17950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.977
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.82))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	96.230	1.620
High resolution limit [Å]	1.580	1.580
Number of reflections	29690	2166
<I/σ(I)>	25.9
Completeness [%]	100.0
Redundancy	38.2
CC(1/2)	1.000	0.803

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2M ammonium sulfate, 0.1M bis-tris pH 5.5