9GOC
Crystal structure of DPP9 Ser730Ala in complex with sulphostin.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.99998 |
| Spacegroup name | P 1 |
| Unit cell lengths | 88.613, 106.531, 121.270 |
| Unit cell angles | 65.15, 70.72, 76.35 |
Refinement procedure
| Resolution | 83.240 - 1.890 |
| R-factor | 0.17691 |
| Rwork | 0.177 |
| R-free | 0.19945 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.321 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC ((Version 1.1.7)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.240 | 1.930 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Number of reflections | 288843 | 14452 |
| <I/σ(I)> | 6 | |
| Completeness [%] | 96.2 | |
| Redundancy | 2.2 | |
| CC(1/2) | 0.990 | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG2000 MME 20% (w/v), 0.1 M Tris pH 6.75 |
