9GLZ
KRas-G12D-GMPPnP in complex with the nanobody KM12-AM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000043 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 84.072, 122.320, 105.615 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.730 - 2.100 |
| R-factor | 0.241 |
| Rwork | 0.239 |
| R-free | 0.27730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1AKNG |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.930 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.160 | 1.880 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.078 | 1.570 |
| Number of reflections | 43995 | 2200 |
| <I/σ(I)> | 14.3 | 1.4 |
| Completeness [%] | 94.1 | 56.4 |
| Redundancy | 10.1 | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 30% PEG 1000, 0.2 M LiNO3 |
