9GLE

Jumonji domain-containing protein 2A with crystallization epitope mutations A91T:T93S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976
Spacegroup name	C 1 2 1
Unit cell lengths	117.700, 90.780, 90.670
Unit cell angles	90.00, 107.19, 90.00

Refinement procedure

Resolution	40.449 - 1.880
Rwork	0.185
R-free	0.22230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	2.053
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.77))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.449	1.930
High resolution limit [Å]	1.880	1.880
Number of reflections	73694	5432
<I/σ(I)>	12.5
Completeness [%]	99.5
Redundancy	3.4
CC(1/2)	0.997	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M lithium sulfate, 25% PEG3350, 0.1M bis-tris pH 6.5