9GI4
TFIIIC5 DNA binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95374 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.524, 37.644, 276.677 |
| Unit cell angles | 90.01, 90.02, 59.90 |
Refinement procedure
| Resolution | 46.110 - 2.630 |
| R-factor | 0.2463 |
| Rwork | 0.244 |
| R-free | 0.29960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.486 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.110 | 2.742 |
| High resolution limit [Å] | 2.630 | 2.670 |
| Rpim | 0.070 | 0.692 |
| Number of reflections | 38159 | 3706 |
| <I/σ(I)> | 10 | |
| Completeness [%] | 97.9 | 93.68 |
| Redundancy | 3.6 | |
| CC(1/2) | 0.995 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293.15 | 0.1 M Phosphate/Citrate pH 4.2, 40% PEG 300. |
