9GHJ

Junin virus GP1-GP2 heterodimer in complex with Fab of JUN1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-23
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	C 1 2 1
Unit cell lengths	225.789, 71.974, 80.231
Unit cell angles	90.00, 95.89, 90.00

Refinement procedure

Resolution	79.810 - 2.090
R-factor	0.214
Rwork	0.212
R-free	0.24710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.555
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	112.300	2.130
High resolution limit [Å]	2.090	2.090
Number of reflections	76048	3801
<I/σ(I)>	6.6
Completeness [%]	100.0
Redundancy	7
CC(1/2)	0.993	0.318

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.65	20% v/v 2-Propanol, 0.1 M Tris pH 8.0, 5% w/v PEG 8,000, 6% 2-Methyl-2,4-pentanediol