9GFE
hRAR LBD protein in complex with AM580 agonist ligand and a stapled peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-20 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.660, 62.340, 49.470 |
| Unit cell angles | 90.00, 105.30, 90.00 |
Refinement procedure
| Resolution | 28.170 - 1.583 |
| R-factor | 0.2057 |
| Rwork | 0.204 |
| R-free | 0.22910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.600 | 1.680 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.028 | 0.531 |
| Number of reflections | 33762 | 5222 |
| <I/σ(I)> | 17.5 | |
| Completeness [%] | 96.3 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | PEG 3350 20%, 0.2M Lithium Acetate, 0.05M NH4 Citrate |
