9GCP

ChREBP/14-3-3 complex stabilized by AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	58.515, 80.279, 244.276
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.600 - 2.590
R-factor	0.2301
Rwork	0.228
R-free	0.27130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.948
Data reduction software	XDS
Data scaling software	pointless
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.600	2.630
High resolution limit [Å]	2.590	2.590
Number of reflections	68910	2618
<I/σ(I)>	9.97
Completeness [%]	99.7
Redundancy	7
CC(1/2)	0.990	0.270

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	50 mM MES ph 6.0 2.2M Sodium Malonate