9GCH
Human mitochondrial RNase Z with tRNA-His-CCA, SDR5C1/TRMT10C focus
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 1.9 |
| Number of Particle | 4773315 |
| Software | Warp,RELION,PHENIX,Coot,cryoSPARC |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
