9GBF

X-RAY structure of PHDvC5HCH tandem domain of NSD2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.998
Spacegroup name	P 1 21 1
Unit cell lengths	36.131, 45.249, 60.646
Unit cell angles	90.00, 99.65, 90.00

Refinement procedure

Resolution	4.970 - 1.760
R-factor	0.1918
Rwork	0.188
R-free	0.22810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.607
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.000	1.773
High resolution limit [Å]	1.601	1.601
Number of reflections	17296	865
<I/σ(I)>	9.7
Completeness [%]	87.3
Redundancy	3.3
CC(1/2)	0.997	0.483

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	28% PEG Smear Medium, 0.1M HEPES, pH 7.5, 0.05M magnesium sulfate