9G9N

Lipid III flippase WzxE with NB10 and NB7 nanobodies in inward-facing conformation - crystal 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-07
Detector	DECTRIS EIGER2 X CdTe 9M
Wavelength(s)	0.6199
Spacegroup name	C 2 2 21
Unit cell lengths	84.240, 191.910, 101.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.400 - 2.800
R-factor	0.2587
Rwork	0.256
R-free	0.31330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.495
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.800	2.850
High resolution limit [Å]	2.800	2.800
Number of reflections	20612	1002
<I/σ(I)>	6.5	1.3
Completeness [%]	99.9	100
Redundancy	6.8	6.9
CC(1/2)	1.000	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5	293	tri-sodium citrate, lithium sulphate, sodium chloride, PEG400, E coli polar lipids, monoolein