9G2J
Thaumatin structure determined using SoS chip at ID29 (serial crystallography)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2023-01-01 |
| Detector | PSI JUNGFRAU 4M |
| Wavelength(s) | 1.073 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.849, 57.849, 150.328 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.904 - 1.690 |
| Rwork | 0.158 |
| R-free | 0.16880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.751 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 1.800 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Number of reflections | 29237 | 1412 |
| <I/σ(I)> | 15.1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 4475 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7 | 293 | 0.8 M NA, K TARTRATE, 0.1 M NA HEPES |
