Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-26 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.999999 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.140, 157.670, 179.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.370 - 2.300 |
| R-factor | 0.2021 |
| Rwork | 0.200 |
| R-free | 0.23580 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.512 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (1.20_4459) |
| Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.370 | 2.320 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.193 | 4.220 |
| Rmeas | 0.201 | 4.390 |
| Rpim | 0.055 | 1.200 |
| Number of reflections | 131347 | 12597 |
| <I/σ(I)> | 11.17 | 0.56 |
| Completeness [%] | 99.3 | 94.25 |
| Redundancy | 13.3 | |
| CC(1/2) | 0.999 | 0.209 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 5% PEG 4K, 8% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine |
