9FWH
Crystal Structure of SARS-CoV-2 NSP10-ExoN in complex with VT00019
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.038, 70.679, 109.382 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.360 - 2.350 |
R-factor | 0.2213 |
Rwork | 0.220 |
R-free | 0.24740 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.007 |
RMSD bond angle | 0.930 |
Data reduction software | XDS (Jan 10, 2022) |
Data scaling software | Aimless (0.7.7) |
Refinement software | BUSTER (2.10.4) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 59.364 | 59.364 | 2.396 |
High resolution limit [Å] | 2.350 | 6.391 | 2.355 |
Rmerge | 0.107 | 0.046 | 1.337 |
Rmeas | 0.112 | 0.049 | 1.388 |
Rpim | 0.031 | 0.014 | 0.371 |
Total number of observations | 13792 | 13943 | |
Number of reflections | 20366 | 1129 | 1010 |
<I/σ(I)> | 16.12 | 42.3 | 2.14 |
Completeness [%] | 99.2 | 99.7 | 100 |
Redundancy | 13.33 | 12.22 | 13.8 |
CC(1/2) | 0.998 | 0.998 | 0.786 |
Anomalous redundancy | 7.3 | 7.2 | |
CC(ano) | 0.225 | -0.027 | |
|DANO|/σ(DANO) | 1.4 | 0.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Morpheus buffer 2 (pH: 7.5): 0.10M - Morpheus Ethylene glycols: 24.50%w/v Morpheus Amino acids: 0.09M |