9FQ1

Structure of the disease-causing mutant P20S of human KCTD1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-30
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	68.053, 96.003, 116.805
Unit cell angles	90.00, 98.20, 90.00

Refinement procedure

Resolution	14.992 - 2.600
R-factor	0.196
Rwork	0.194
R-free	0.24310
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.944
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.660
High resolution limit [Å]	2.600	2.600
Number of reflections	52034	2616
<I/σ(I)>	6.8
Completeness [%]	99.6
Redundancy	3.4
CC(1/2)	0.995	0.379

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.8 M sodium chloride, 0.1 M sodium phosphate monobasic monohydrate, 0.1 M Potassium phosphate monobasic, 0.1 M Mes monohydrate (pH 6.5).