EM specimen
Vitrification | Yes |
Instrument | LEICA EM GP |
EM Data Collection
Microscope | FEI TALOS ARCTICA |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.58 |
Number of Particle | 14445 |
Software | cryoSPARC,RELION,PHENIX,EPU,UCSF ChimeraX |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |