9FJH

Two PLK1 PBD proteins bound to CENP-U(58-114) phosphorylated at Thr78 and Thr98

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9998416639211805
Spacegroup name	P 21 21 2
Unit cell lengths	84.890, 135.090, 60.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.450 - 2.150
R-factor	0.218
Rwork	0.216
R-free	0.24970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.488
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.450	36.220	2.210
High resolution limit [Å]	2.150	9.620	2.150
Rmerge	0.082	0.039	0.082
Rmeas	0.085	0.041	0.169
Number of reflections	38912	506	2810
<I/σ(I)>	16.84
Completeness [%]	100.0
Redundancy	13.5
CC(1/2)	0.999	0.999	0.571

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	293	0.2M NaCl, 0.1 M CHES pH9.5, 10 v/v% PEG8000