9FGG

Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in Saposin A nanodisc bound to GABA and Etomidate in the long-lived symmetric desensitised state

This is a non-PDB format compatible entry.

EM specimen

Vitrification	Yes
Instrument	LEICA EM GP

EM Data Collection

Microscope	TFS KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Energy Filter	GIF Bioquantum
Detector	GATAN K3 (6k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.6
Number of Particle	2852819
Software	Warp,cryoSPARC,RELION,EPU,CTFFIND,PHENIX
Symmetry	C1
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION