9FCJ
USP1 bound to ML323 and ubiquitin conjugated to FANCD2 (ordered subset, focused refinement)
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | TFS KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.7 |
Number of Particle | 6716868 |
Software | cryoSPARC,PHENIX,Coot,ISOLDE |
Symmetry | C1 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |