9F6G

Human USP30 chimera bound to Ubiquitin-PA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-25
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 21 21 21
Unit cell lengths	46.639, 50.706, 156.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.700 - 1.500
R-factor	0.1843
Rwork	0.183
R-free	0.21070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.331
Data reduction software	DIALS (3.19.0-g3823b7bec-release)
Data scaling software	Aimless (0.7.15)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.640	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.073	1.240
Number of reflections	58606	2821
<I/σ(I)>	10.7	1.4
Completeness [%]	97.1	96.8
Redundancy	6.2	6.4
CC(1/2)	0.998	0.426

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.56 M sodium citrate pH 7.0