9F6B
Human neuropilin-1 in a complex with a quinoline based antagonists
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-28 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.570, 92.220, 40.830 |
| Unit cell angles | 90.00, 94.84, 90.00 |
Refinement procedure
| Resolution | 20.510 - 1.570 |
| R-factor | 0.19534 |
| Rwork | 0.193 |
| R-free | 0.23905 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.344 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.510 | 1.600 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.040 | 0.210 |
| Number of reflections | 41294 | 1640 |
| <I/σ(I)> | 14.3 | |
| Completeness [%] | 99.0 | |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2 M Ammonium Chloride, 14% PEG3350 |
