9F1I
Crystal structure of a first-in-class antibody for alpha-1,6-fucosylated prostate-specific antigen, target bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2021-09-10 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.310, 87.395, 150.427 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.200 - 1.380 |
| R-factor | 0.1564 |
| Rwork | 0.155 |
| R-free | 0.18650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.766 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207+SVN) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.240 | 1.490 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmeas | 0.054 | 1.169 |
| Number of reflections | 155757 | 7791 |
| <I/σ(I)> | 15.7 | 1.5 |
| Completeness [%] | 95.7 | 65.4 |
| Redundancy | 6.7 | 7.3 |
| CC(1/2) | 0.999 | 0.633 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 100 mM Tris-HCl pH 8.5, 6.25% v/v PEG 3350, 6.25% v/v PEG 4000, 6.25% v/v PEG 2000 and 6.25% v/v PEG 5000 MME |
