9EZH

Structure of single-domain antibody VHH_h4 in complex with human Vsig4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	80
Detector technology	PIXEL
Collection date	2023-07-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97926
Spacegroup name	P 21 21 21
Unit cell lengths	25.764, 50.961, 175.238
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.930 - 1.940
R-factor	0.1963
Rwork	0.194
R-free	0.23940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.057
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20_4459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.619	1.971
High resolution limit [Å]	1.938	1.938
Rmerge	0.096	1.094
Number of reflections	18076	928
<I/σ(I)>	16	2.5
Completeness [%]	100.0
Redundancy	12.6
CC(1/2)	0.998	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0,1M Bis-Tris pH 6.0 and 24% PEG3350