9EY8
Crystal structure of human tyrosinase-related protein 1 (TYRP1) in complex with (s)-amino-L-tyrosine
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-08 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.965459 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.972, 126.973, 212.339 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.260 - 2.200 |
| R-factor | 0.1913 |
| Rwork | 0.189 |
| R-free | 0.23270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.028 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.260 | 2.220 |
| High resolution limit [Å] | 2.198 | 2.200 |
| Rmerge | 0.089 | 1.377 |
| Rmeas | 0.105 | |
| Rpim | 0.056 | 0.927 |
| Number of reflections | 234158 | 6894 |
| <I/σ(I)> | 8.29 | 0.59 |
| Completeness [%] | 99.5 | 88.35 |
| Redundancy | 3.4 | 2.9 |
| CC(1/2) | 0.996 | 0.395 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | Sodium bromide, Bis-Tris propane, PEG 3350 |
