9EY8

Crystal structure of human tyrosinase-related protein 1 (TYRP1) in complex with (s)-amino-L-tyrosine

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-08
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.965459
Spacegroup name	P 21 21 21
Unit cell lengths	88.972, 126.973, 212.339
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.260 - 2.200
R-factor	0.1913
Rwork	0.189
R-free	0.23270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.028
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.260	2.220
High resolution limit [Å]	2.198	2.200
Rmerge	0.089	1.377
Rmeas	0.105
Rpim	0.056	0.927
Number of reflections	234158	6894
<I/σ(I)>	8.29	0.59
Completeness [%]	99.5	88.35
Redundancy	3.4	2.9
CC(1/2)	0.996	0.395

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	Sodium bromide, Bis-Tris propane, PEG 3350