9EWL
The sTeLIC pentameric Ligand-Gated Ion Channel (wild-type) in complex with 4-bromoamphetamine
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-18 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.919 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 218.380, 114.320, 142.440 |
| Unit cell angles | 90.00, 113.05, 90.00 |
Refinement procedure
| Resolution | 24.001 - 3.200 |
| Rwork | 0.215 |
| R-free | 0.26020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.466 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.314 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 33187 | 1016 |
| <I/σ(I)> | 15.11 | |
| Completeness [%] | 62.0 | |
| Redundancy | 2 | |
| CC(1/2) | 0.980 | 0.724 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM Tris pH 8.0; 150 mM MgCl2; 3% DMSO; 32% PEG 200 |
