9EWG

DNA Polymerase Lambda I493K, TTP:At Ca2+ Ground State Ternary Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 21 21 21
Unit cell lengths	56.042, 62.504, 141.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.700 - 2.000
R-factor	0.2083
Rwork	0.205
R-free	0.25170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.733
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.700	2.118
High resolution limit [Å]	1.916	1.916
Rpim	0.052	0.807
Number of reflections	25444	1272
<I/σ(I)>	11	1.7
Completeness [%]	93.3
Redundancy	11.9
CC(1/2)	0.996	0.504

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277.15	20 mM bicine pH7.5 300 mM Na-K tartrate 17.5% PEG 20K 10 mM praseodyme acetate