9EWB

DNA Polymerase Lambda I493R, TTP:At Ca2+ Ground State Ternary Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-27
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980112
Spacegroup name	P 21 21 21
Unit cell lengths	56.280, 62.510, 140.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.830 - 2.320
R-factor	0.2056
Rwork	0.204
R-free	0.22490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.402
Data reduction software	ADDREF
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.740	2.380
High resolution limit [Å]	2.320	2.320
Rmerge	0.102	1.640
Number of reflections	22111	3488
<I/σ(I)>	17.85
Completeness [%]	99.9
Redundancy	13.4
CC(1/2)	0.990	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277.15	20 mM bicine pH 7,5, 300 mM Na-K tartrate, 22,5% PEG Smear high