9EW7
Binary structure of 14-3-3s and CRAF phosphopeptide (pS259)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU MICROMAX-003 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-05 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.541870 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.375, 112.196, 62.708 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.430 - 1.800 |
| R-factor | 0.16526 |
| Rwork | 0.164 |
| R-free | 0.19251 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.293 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PDB-REDO |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.430 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 27325 | 1612 |
| <I/σ(I)> | 15.3 | 4.5 |
| Completeness [%] | 100.0 | |
| Redundancy | 6.2 | |
| CC(1/2) | 0.998 | 0.932 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.095 M HEPES pH=7.1-7.7 0.19 M CaCl2 5% glycerol 24-29% PEG400 |
