9EVJ

Crystal Structure of human Collagen Hydroxylysine Galactosyltransferase GLT25D1/COLGALT1: complex with Mn2+ and UDP-Gal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9999
Spacegroup name	I 2 3
Unit cell lengths	220.855, 220.855, 220.855
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.380 - 2.700
R-factor	0.1873
Rwork	0.185
R-free	0.22250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.532
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20_4459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.380	2.790
High resolution limit [Å]	2.700	2.700
Rmerge	0.110	1.251
Rpim	0.058	0.920
Number of reflections	49018	4444
<I/σ(I)>	13.5
Completeness [%]	99.7	97.9
Redundancy	8.7	5.2
CC(1/2)	0.998	0.395

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	8% poly-ethylene-glycol (PEG) MW 4000, 100 mM 2-(N-morpholino)ethanesulfonic acid (MES)/NaOH, 20% glycerol, pH 6.5